dos.cuatro Illumina series processing and you can microbiota analysis investigation
To own larval samples the whole muscles was applied getting DNA isolation. In total, 118 private bravery out-of people and you can nine larval trials was processed. DNA separation try performed having private samples by using the ZymoBIOMICS 96 DNA Kits (Zymo Browse) together with bead beating at the 3200 rpm to possess 15 min on a beneficial grant MPS-step one multiplate shaker (Offer Products). Bad extraction control (NECs) also mock-neighborhood positive regulation (Zymo Look) was basically integrated.
Bumble bees have been taken out of the new freezer and you may dissected using flame sterilized tweezers to get the whole abdomen also harvest, foregut and you will hindgut
I put a twin-indexing method of enhance brand new V4 area of the 16S rRNA gene because done by Kozich mais aussi al. (2013). …